New PrePrint: Testing the potential of 16S for DNA metabarcoding

Together with our colleges from France we just have published our first PrePrint, where we test the potential of the 16S marker for DNA metabarcoding. We were able to show that freshwater insects are more consistently amplified with the developed 16S primer than with standard Folmer COI barcoding primer. While the taxonomic resolution of the 16S marker remains unknown for insects, the ribosomal marker might be a viable alternative metabarcoding marker when local reference databases can be easily established. For now we will however continue using the COI marker, due to availability of good reference databases and optimised freshwater COI markers which show similar performance.



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